Mel Blanc provided the voices for Bugs Bunny, Red Hot Ryder, and some of the villagers. Robert C. Bruce was the opening narrator. Lou Lilly wrote the story, and Leon Schlesinger served as producer. While only Manny Gould was credited as an animator, Robert McKimson, Rod Scribner, and Jack Bradbury also aided in the process. Other uncredited 'staff' includes the composers of several uncredited bits of non-original music--Sanford Faulkner ('Arkansas Traveller'), M.K. Jerome ('My Little Buckaroo', where the title ostensibly takes its name), Gioacchino Rossini ('William Tell Overture'), Franz Schubert ('Der Erlkönig'), and J.S. Zamecnik ('In the Stirrups'). All original music was composed by Carl W. Stalling
Unlike most shorts, Bugs Bunny serves as antagonist. In the cartoon, he plays a carrot thief called the Masked Marauder, whom Brooklyn's "Red Hot Ryder" (a parody of Red Ryder) must bring to justice. The cartoon portrays Red Hot Ryder as a dimwit who cannot distinguish Bugs Bunny from the Masked Marauder, and his good-natured slowness is consistently mocked: When Bugs Bunny as the Masked Marauder threatens to shoot Red Hot Ryder, saying, "Stick 'em up, or I'll blow your brains out," the latter treats it like a choice, replying, "Well, now, that's mighty neighborly of you." In the end, Red Hot Ryder catches on, but is unable to catch the Masked Marauder, in the end he tricks him into jumping into the Grand Canyon, when underground Red Hot Ryder finally figures out that Bugs is the Masked Marauder. Bugs pops up from beneath the ground with a lit candle and says "That's right! That's right! You win the 64 dollar question!" (a reference to the "big prize" on the famous radio quiz show, Take It or Leave It. He then kisses him and blows out the candle.